Publications

42. “High-contrast fluorescence detection of metastatic breast cancer including bone and liver micrometastases via size-controlled pH-activatable water-soluble probes.” Hu Xiong, Hao Zuo, Yunfeng Yan, Gino Occhialini, Kejin Zhou, Yihong Wan, and Daniel J. Siegwart* Advanced Materials, 2017, 29, 1700131.

41. Non-viral CRISPR/Cas gene editing in vitro and in vivo enabled by synthetic nanoparticle co-delivery of Cas9 mRNA and sgRNA.” Jason B. Miller, Shuyuan Zhang, Petra Kos, Hu Xiong, Kejin Zhou, Sofya S. Perelman, Hao Zhu,  and Daniel J. Siegwart* Angewandte Chemie International Edition, 2017, 56, 1059-1063.
Highlighted in C&EN’s cover story on CRISPR/Cas delivery.

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40. HDAC inhibitor conjugated polymeric prodrug micelles for doxorubicin delivery.” Suchithra A. Senevirathne, Katherine E. Washington, Jason B. Miller, Michael C. Biewer, David Oupicky,* Daniel J. Siegwart,* and Mihaela C. Stefan* Journal of Materials Chemistry B, 2017, 5, 2106-2114.

39. Gpr132 sensing of lactate mediates tumor-macrophage interplay to promote breast cancer metastasis.” Peiwen Chen, Hao Zuo, Hu Xiong, Matthew J. Kolar, Qian Chu, Alan Saghatelian, Daniel J. Siegwart, and Yihong Wan.* Proceedings of the National Academy of Sciences, U.S.A., 2017, 114, 580-585.

paper3738. Aerosol delivery of stabilized polyester-siRNA nanoparticles to silence gene expression in orthotopic lung tumors.” Yunfeng Yan, Kejin Zhou, Hu Xiong, Jason B. Miller, Edward A. Motea, David A. Boothman, Li Liu,* and Daniel J. Siegwart*  Biomaterials, 2017, 118, 84-93.

paper3637. Functional polyesters enable selective siRNA delivery to lung cancer over matched normal cells.” Yunfeng Yan, Li Liu, Hu Xiong, Jason B. Miller, Kejin Zhou, Petra Kos, Kenneth E. Huffman, Sussana Elkassih, John W. Norman, Ryan Carstens, James Kim, John D. Minna, and Daniel J. Siegwart.*  Proceedings of the National Academy of Sciences, U.S.A.2016, 113, E5702–E5710.

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36. Intercalation-mediated nucleic acid nanoparticles for siRNA delivery. Kejin Zhou, Petra Kos, Yunfeng Yan, Hu Xiong, Yi-Li Min, Jonathan T. Minnig, Jason B. Miller, and Daniel J. Siegwart.* Chemical Communications, 2016, 52, 12155-12158.

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35. Progress towards the synthesis of amino polyesters via ring-opening polymerization (ROP) of functional lactones.” Jing Hao, Sussana Elkassih, and Daniel J. Siegwart.* Synlett, 2016, 27, 2285-2292.

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34. Activatable water soluble probes enhance tumor imaging by responding to dysregulated pH and exhibiting high tumor-to-liver fluorescence emission contrast.” Hu Xiong, Petra Kos, Yunfeng Yan, Kejin Zhou, Jason B. Miller, Sussana Elkassih, and Daniel J. Siegwart* Bioconjugate Chemistry, 2016, 27, 1737–1744.

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33. “Modular degradable dendrimers enable small RNAs to extend survival in an aggressive liver cancer model.” Kejin Zhou, Liem H. Nguyen, Jason B. Miller, Yunfeng Yan, Petra Kos, Hu Xiong, Lin Li, Jing Hao, Jonathan T. Minnig, Hao Zhu, and Daniel J. Siegwart.* Proceedings of the National Academy of Sciences, U.S.A., 2016, 113, 520-525.

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32. “Tumor imaging based on photon upconversion of Pt(II) porphyrin rhodamine co-modified NIR excitable cellulose enhanced by aggregation.” Atsushi Nagai,* Jason B. Miller, Petra Kos, Sussana Elkassih, Hu Xiong, and Daniel J. Siegwart*  ACS Biomaterials Science & Engineering, 2015, 1, 1206-1210.

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31. “Precise regulation of let-7 levels balance organ regeneration against tumor suppression.”  Linwei Wu, Liem H. Nguyen, Kejin Zhou, Yvanka de Soysa, Lin Li, Jason B. Miller, Jianmin Tian, Joseph Locker, Shuyuan Zhang, Gen Shinoda, Marc T. Seligson, Lauren R. Zeitels, Asha Acharya, Sam C. Wang, Joshua T. Mendell, Jinsuke Nishino, Sean J. Morrison, Daniel J. Siegwart, George Q. Daley, Ng Shyh-Chang, and Hao Zhu.* eLife, 2015, 4, e09431. 

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30.  “Rapid synthesis of a lipocationic polyester library via ring-opening polymerization of functional valerolactones for efficacious siRNA delivery.”  Jing Hao, Petra Kos, Kejin Zhou, Jason B. Miller, Lian Xue, Yunfeng Yan, Hu Xiong, Sussana Elkassih, and Daniel J. Siegwart.*  Journal of the American Chemical Society, 2015, 137, 9206-9209.

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29. “Biocompatible organic charge transfer complex nanoparticles based on a semi-crystalline cellulose template.”  Atsushi Nagai,* Jason B. Miller, Jia Du, Petra Kos, Mihaela C. Stefan, and Daniel J. Siegwart.*  Chemical Communications, 2015, 15, 11868-11871.

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28. “One-pot synthesis of functional poly(amino ester sulfide)s and utility in delivering pDNA and siRNA.”  Yunfeng Yan, Lian Xue, Jason B. Miller, Kejin Zhou, Petra Kos, Sussana Elkassih, Atsushi Nagai, Hu Xiong, and Daniel J. Siegwart.*  Polymer, 2015, 72, 271-280.

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27.Fine-tuning thermoresponsive functional poly(ε-caprolactone)s to enhance micelle stability and drug loading.”  Elizabeth A. Rainbolt, Jason B. Miller, Katherine E. Washington, Suchithra A. Senevirathne, Michael C. Biewer, Daniel J. Siegwart,* Mihaela C. Stefan.*  Journal of Materials Chemistry B, 2015, 3, 1779-1787.

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26. “Polymers for drug delivery.” Daniel J. Siegwart.* McGraw-Hill 2015 Yearbook of Science & Technology. New York: McGraw-Hill, 2015 (book chapter).

25. “Scalable synthesis and derivation of functional polyesters bearing ene and epoxide side chains.” Yunfeng Yan, Daniel J. Siegwart.* Polymer Chemistry, 2014, 5, 1362-1371.

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Siegwart, DJ: Postdoctoral research at MIT.

24. “Lipopeptide nanoparticles for potent and selective siRNA delivery in rodents and nonhuman primates.” Yizhou Dong, Kevin T. Love, J. Robert Dorkin, Sasilada Sirirungruang, Yunlong Zhang, Delai Chen, Roman L. Bogorad, Hao Yin, Yi Chen, Arturo J. Vegas, Christopher A. Alabi, Gaurav Sahay, Karsten T. Olejnik, Weiheng Wang, Avi Schroeder, Abigail K. R. Lytton-Jean, Daniel J. Siegwart, Akin Akinc, Carmen Barnes, Scott A. Barros, Mary Carioto, Kevin Fitzgerald, Julia Hettinger, Varun Kumar, Tatiana I. Novobrantseva, June Qin, William Querbes, Victor Koteliansky, Robert Langer, Daniel G. Anderson.* Proceedings of the National Academy of Sciences, 2014, 111, 3955-3960.

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23. “Biophysical properties of degradable polymeric nanoparticles: Effect of molecular weight of amine end-modified poly(ß-amino ester)s on gene delivery efficiency and toxicity.” Ahmed A. Eltoukhy, Daniel J. Siegwart, Christopher A. Alabi, Jay S. Rajan, Robert Langer, Daniel G. Anderson.* Biomaterials, 2012, 33, 3594-3603.

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22. “Automated ARGET ATRP accelerates catalyst optimization for the synthesis of thiol-functionalized polymers.” Daniel J. Siegwart, Matthias Leiendecker, Robert Langer, Daniel G. Anderson.* Macromolecules, 2012, 45, 1254-1261.

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21. “Combinatorial synthesis of chemically diverse core-shell nanoparticles for intracellular delivery.” Daniel J. Siegwart, Kathryn A. Whitehead, Lutz Nuhn, Gaurav Sahay, Hao Cheng, Shan Jiang, Minglin Ma, Abigail Lytton-Jean, Arturo Vegas, Patrick Fenton, Christopher G. Levins, Kevin T. Love, Haeshin Lee, Christina Cortez, Sean P. Collins, Ying Fei Li, Janice Jang, William Querbes, Christopher Zurenko, Tatiana Novobrantseva, Robert Langer, Daniel G. Anderson.* Proceedings of the National Academy of Sciences, 2011, 108, 12996-13001.

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20. “Development of cationic polymer coatings to regulate foreign-body responses.” Minglin Ma, Wendy F. Liu, Paulina S. Hill, Kaitlin M. Bratlie, Daniel J. Siegwart, Justin Chin, Miri Park, Joao Guerreiro, Daniel G. Anderson.* Advanced Materials, 2011, 23, H189-H194.

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19. “A novel family of biodegradable poly(ester amide) elastomers.” Hao Cheng, Paulina S. Hill, Daniel J. Siegwart, Nathaniel Vacanti, Abigail K. R. Lytton-Jean, Seung-Woo Cho, Anne Ye, Robert Langer, Daniel G. Anderson.* Advanced Materials, 2011, 12, H95-H100.

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18. “An injectable thiol-acrylate poly(ethylene glycol) hydrogel.” Christopher D. Pritchard, Timothy M. O’Shea, Daniel J. Siegwart, Eliezer Calo, Daniel G. Anderson, Francis M. Reynolds, John A. Thomas, Jonathan R. Slotkin, Eric J. Woodard, Robert Langer.* Biomaterials, 2010, 32, 587-597.

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17. “Nanoparticulate cellular patches for cell-mediated tumoritropic delivery.” Hao Cheng, Christian J. Kastrup, Renuka Ramanathan, Daniel J. Siegwart, Minglin Ma, Said R. Bogatyrev, Qiaobing Xu, Kathryn A. Whitehead, Robert Langer, Daniel G. Anderson.* ACS Nano, 2010, 4, 625-631.


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Siegwart, DJ: Graduate research at CMU.

16. “ATRP in the design of functional materials for biomedical applications.” Daniel J. Siegwart, Jung Kwon Oh, Krzysztof Matyjaszewski.* Progress in Polymer Science, 2012, 37, 18-37.

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15. “Preparation of Cationic Nanogels for Nucleic Acid Delivery.” Saadyah E. Averick, Eduardo Paredes Ainara Irastorza, Arun R. Shrivats, Abiraman Srinivasan, Daniel J. Siegwart, Andrew J. Magenau, Hong Y. Cho, Eric Hsu, Amram A. Averick, Jinku Kim, Shiguang Liu, Jeffrey O. Hollinger, Subha R. Das, Krzysztof Matyjaszewski.* Biomacromolecules, 2012, 13, 3445-3449.

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14. “Rapid cellular internalization of multifunctional star polymers prepared by Atom Transfer Radical Polymerization.” Hong Y. Cho, Haifeng Gao, Abiraman Srinivasan, Joanna Hong, Sidi A. Bencherif, Daniel J. Siegwart, Hyun-jong Paik, Jeffrey O. Hollinger, Krzysztof Matyjaszewski.* Biomacromolecules, 2010, 11, 2199-2203.

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13. “Nanostructured hybrid hydrogels prepared by a combination of atom transfer radical polymerization and free radical polymerization.” Sidi A. Bencherif, Daniel J. Siegwart, Abiraman Srinivasan, Ferenc Horkay, Jeffrey O. Hollinger, Newell R. Washburn, Krzysztof Matyjaszewski.* Biomaterials, 2009, 30, 5270-5278.

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12. “Cellular uptake of functional nanogels prepared by inverse miniemulsion ATRP with encapsulated proteins, carbohydrates, and gold nanoparticles.” Daniel J. Siegwart, Abiraman Srinivasan, Sidi A. Bencherif, Anuradha Karunanidhi, Jung Kwon Oh, Swaroopa Vaidya, Rongchao Jin, Jeffrey O. Hollinger, Krzysztof Matyjaszewski.* Biomacromolecules, 2009, 10, 2300-2309.

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11. “Cell-adhesive star polymers prepared by ATRP.” Sidi A. Bencherif, Haifeng Gao, Abiraman Srinivasan, Daniel J. Siegwart, Jeffrey O. Hollinger,* Newell R. Washburn,* Krzysztof Matyjaszewski.* Biomacromolecules, 2009, 10, 1795-1803.

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10. “The development of microgels/nanogels for drug delivery applications.” Jung Kwon Oh, Ray Drumright, Daniel J. Siegwart, Krzysztof Matyjaszewski.* Progress in Polymer Science, 2008, 33, 448-477.

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9. “Biotin-, pyrene- and GRGDS-functionalized ATRP polymers and nanogels via end group modification.” Daniel J. Siegwart, Jung Kwon Oh, Haifeng Gao, Sidi A. Bencherif, Fabien Perineau, Andrew K. Bohaty, Jeffrey O. Hollinger, Krzysztof Matyjaszewski.* Macromolecular Chemistry and Physics, 2008, 209, 2179-2193.

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8. “Synthesis, characterization, and in vitro cell culture viability of degradable poly(N-isopropylacrylamide-co-5,6-benzo-2-methylene-1,3-dioxepane)-based polymers and crosslinked gels.” Daniel J. Siegwart, Sidi A. Bencherif, Abiraman Srinivasan, Jeffrey O. Hollinger, Krzysztof Matyjaszewski.* Journal of Biomedical Materials Research Part A, 2008, 87, 345-358.

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7. “Synthesis and biodegradation of nanogels as delivery carriers for carbohydrate drugs.” Jung Kwon Oh, Daniel J. Siegwart, Krzysztof Matyjaszewski.* Biomacromolecules, 2007, 8, 3326-3331.

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6. “Biodegradable nanogels prepared by atom transfer radical polymerization as potential drug delivery carriers: synthesis, biodegradation, in vitro release, and bioconjugation.” Jung Kwon Oh, Daniel J. Siegwart, Hyung-il Lee, Gizelle Sherwood, Linda Peteanu, Jeffrey O. Hollinger, Kazunori Kataoka, Krzysztof Matyjaszewski.* Journal of the American Chemical Society, 2007, 129, 5939-5945.

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5. “Solvent induced morphologies of poly(methyl methacrylate-b-ethylene oxide-b-methyl methacrylate) triblock copolymers synthesized by atom transfer radical polymerization.” Daniel J. Siegwart, Wei Wu, Monisha Mandalaywala, Magi Tamir, Traian Sarbu, Michael S. Silverstein, Tomasz Kowalewski, Jeffrey O. Hollinger, Krzysztof Matyjaszewski.* Polymer, 2007, 48, 7279-7290.

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4. “Synthesis and characterization of styrene/butyl acrylate linear and star block copolymers via Atom Transfer Radical Polymerization.” Jinyu Huang, Shijun Jia, Daniel J. Siegwart, Tomasz Kowalewski, Krzysztof Matyjaszewski.* Macromolecular Chemistry and Physics, 2006, 207, 801-811.

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3. “Characterization of α-,ω-dihydroxypolystyrene by gradient polymer elution chromatography and two-dimensional liquid chromatography.” Haifeng Gao, Daniel J. Siegwart, Nazeem Jahed, Traian Sarbu, Krzysztof Matyjaszewski.* Designed Monomers and Polymers, 2005, 8, 533-546.

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2. “Synthesis of hydroxy-telechelic poly(methyl acrylate) and polystyrene by Atom Transfer Radical Coupling.” Traian Sarbu, Koon-Yee Lin, James Spanswick, Roberto R. Gil, Daniel J. Siegwart, Krzysztof Matyjaszewski.* Macromolecules, 2004, 37, 9694-9700.

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1. “Polystyrene with designed molecular weight distribution by Atom Transfer Radical Coupling.” Traian Sarbu, Koon-Yee Lin, John Ell, Daniel J. Siegwart, James Spanswick, Krzysztof Matyjaszewski.* Macromolecules, 2004, 37, 3120-3127.

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