Publications

87. “In vivo editing of lung stem cells for durable gene correction in mice.” Yehui Sun,# Sumanta Chatterjee,# Xizhen Lian, Zachary Traylor, Sandhya R. Sattiraju, Yufen Xiao, Sean A. Dilliard, Yun-Chieh Sung, Minjeong Kim, Sang M. Lee, Stephen Moore, Xu Wang, Di Zhang, Shiying Wu, Pratima Basak, Jialu Wang, Jing Liu, Rachel J. Mann, David F. LePage, Weihong Jiang, Shadaan Abid, Mirko Hennig, Anna Martinez, Brandon A. Wustman, David J. Lockhart, Raksha Jain, Ronald A. Conlon, Mitchell L. Drumm, Craig A. Hodges, and Daniel J. Siegwart.* Science, 384, 1196 (2024).

86. “Bone-marrow-homing lipid nanoparticles for genome editing in diseased and malignant haematopoietic stem cells.” Xizhen Lian, Sumanta Chatterjee, Yehui Sun, Sean A. Dilliard, Stephen Moore, Yufen Xiao, Xiaoyan Bian, Kohki Yamada, Yun-Chieh Sung, Rachel M. Levine, Kalin Mayberry, Samuel John, Xiaoye Liu, Caroline Smith, Lindsay T. Johnson, Xu Wang, Cheng Cheng Zhang, David R. Liu, Gregory A. Newby, Mitchell J. Weiss, Jonathan S. Yen, and Daniel J. Siegwart.* Nature Nanotechnology doi 10.1038/s41565-024-01680-8 (2024).

85. “In situ production and secretion of proteins endow therapeutic benefit against psoriasiform dermatitis and melanoma.” Qiang Cheng, Lukas Farbiak, Amogh Vaidya, Erick Guerrero, Eunice E. Lee, Elysha K. Rose, Xu Wang, Joshua Robinson, Sang M. Lee, Tuo Wei, William E. Miller, Ester Alvarez Benedicto, Xizhen Lian, Richard C. Wang, and Daniel J. Siegwart.* Proceedings of the National Academy of Sciences, U.S.A., 120, e2313009120 (2023).

84. “Modular design of biodegradable ionizable lipids for improved mRNA delivery and precise cancer metastasis delineation in vivo.” Zhaoming Chen, Yang Tian, Jieyu Yang, Fapu Wu, Senyao Liu, Wenwen Cao, Weijia Xu, Tao Hu, Daniel J. Siegwart, and Hu Xiong* Journal of the American Chemical Society, 145, 24302–24314 (2023).

83. “Spleen SORT LNP generated in situ CAR T cells extend survival in a mouse model of lymphoreplete B cell lymphoma.” Ester Álvarez-Benedicto, Zeru Tian, Sumanta Chatterjee, Domenico Orlando, Minjeong Kim, Erick D. Guerrero, Xu Wang, Daniel J. Siegwart.* Angewandte Chemie International Edition, 62, e202310395 (2023).

82. “Lung SORT LNPs enable precise homology-directed repair mediated CRISPR/Cas genome correction in cystic fibrosis models.” Tuo Wei, Yehui Sun, Qiang Cheng, Sumanta Chatterjee, Zachary Traylor, Lindsay T. Johnson, Melissa L. Coquelin, Jialu Wang, Michael J. Torres, Xizhen Lian, Xu Wang, Yufen Xiao, Craig A. Hodges, Daniel J. Siegwart.* Nature Communications, 14, 7322 (2023).

81. “Nanotechnology-based mRNA vaccines.” Shuying Chen, Xiangang Huang, Yonger Xue, Ester Álvarez-Benedicto, Yesi Shi, Wei Chen, Seyoung Koo, Daniel J. Siegwart,* Yizhou Dong,* Wei Tao.* Nature Reviews Methods Primers, 3, 63 (2023).

80. “The interplay of quaternary ammonium lipid structure and protein corona on lung-specific mRNA delivery by selective organ targeting (SORT) nanoparticles.”Sean A. Dilliard, Yehui Sun, Madeline O. Brown, Yun-Chieh Sung, Sumanta Chatterjee, Lukas Farbiak, Amogh Vaidya, Xizhen Lian, Xu Wang, Andrew Lemoff, Daniel J. Siegwart.* Journal of Controlled Release, 361, 361-372 (2023).

79. “In vivo screening identifies SPP2, a secreted factor that negatively regulates liver regeneration.” Yu-Hsuan Lin, Qiyu Zeng, Yuemeng Jia, Zixi Wang, Lin Li, Meng-Hsiung Hsieh, Qiang Cheng, Chase A Pagani, Nicholas Livingston, Jeon Lee, Yu Zhang, Tripti Sharma, Daniel J Siegwart, Dean Yimlamai, Benjamin Levi, and Hao Zhu.* Hepatology, 78, 1133-1148 (2023).

78. “Passive, active and endogenous organ-targeted lipid and polymer nanoparticles for delivery of genetic drugs.” Sean A. Dilliard and Daniel J. Siegwart* Nature Reviews Materials, 8, 282–300 (2023).

77. “Preparation of selective organ-targeting (SORT) lipid nanoparticles (LNPs) using multiple technical methods for tissue-specific mRNA delivery.” Xu Wang, Shuai Liu, Yehui Sun, Xueliang Yu, Sang M. Lee, Qiang Cheng, Tuo Wei, Junyu Gong, Joshua Robinson, Di Zhang, Xizhen Lian, Pratima Basak, and Daniel J. Siegwart* Nature Protocols, 18, 265–291 (2023).

76. “Lipid nanoparticle (LNP) chemistry can endow unique in vivo RNA delivery fates within the liver that alter therapeutic outcomes in a cancer model.” Lindsay T. Johnson, Di Zhang, Kejin Zhou, Sang M. Lee, Shuai Liu, Sean A. Dilliard, Lukas Farbiak, Sumanta Chatterjee, Yu-Hsuan Lin, and Daniel J. Siegwart* Molecular Pharmaceutics, 19, 3973–3986 (2022).

• Selected as ACS Editors’ Choice.

75. “Next-generation Diprovocims with potent human and murine TLR1/TLR2 agonist activity that activate the innate and adaptive immune response.” Ming-Hsiu Yang, Jamie L. Russell, Yuto Mifune, Ying Wang, Hexin Shi, Eva Marie Y. Moresco, Daniel J. Siegwart, Bruce Beutler,* and Dale L. Boger.* Journal of Medicinal Chemistry, 65, 9230-9252 (2022).

74. “Enhancing CRISPR/Cas gene editing through modulating cellular mechanical properties for cancer therapy” Di Zhang, Guoxun Wang, Xueliang Yu, Tuo Wei, Lukas Farbiak, Lindsay T. Johnson, Alan Mark Taylor, Jiazhu Xu, Yi Hong, Hao Zhu, Daniel J. Siegwart.* Nature Nanotechnology, 17, 777–787 (2022).

• Highlighted in a UT Southwestern press release entitled “Lipid nanoparticles carry gene-editing cancer drugs past tumor defenses.” (June 23, 2022).
• Highlighted in The Scientist, “Researchers Develop a CRISPR-Based Therapy That Penetrates Solid Tumors.”

73. “News & Views: Disrupting off-target Cas9 activity in the liver.” Sean A. Dilliard, Daniel J. Siegwart.* Nature Biomedical Engineering, 6, 106–107 (2022).

72. “In vivo CRISPR screening identifies BAZ2 chromatin remodelers as druggable regulators of mammalian liver regeneration.” Yuemeng Jia, Lin Li, Yu-Hsuan Lin, Purva Gopal, Shunli Shen, Kejin Zhou, Xueliang Yu, Tripti Sharma, Yu Zhang, Daniel J. Siegwart, Joseph M. Ready, Hao Zhu.* Cell Stem Cell, 29, 1–14, (2022).

71. “On the mechanism of tissue-specific mRNA delivery by selective organ targeting nanoparticles.” Sean A. Dilliard, Qiang Cheng,* Daniel J. Siegwart.* Proceedings of the National Academy of Sciences, U.S.A., 118, e2109256118 (2021).

70. “Zwitterionic phospholipidation of cationic polymers facilitates systemic mRNA delivery to spleen and lymph nodes.” Shuai Liu, Xu Wang, Xueliang Yu, Qiang Cheng, Lindsay T. Johnson, Sumanta Chatterjee, Di Zhang, Sang M. Lee, Yehui Sun, Ting-Chih Lin, John L. Liu, and Daniel J. Siegwart.* Journal of the American Chemical Society, 143, 21321-21330 (2021).

69. “Optimization of phospholipid chemistry for improved lipid nanoparticle (LNP) delivery of messenger RNA (mRNA).” Ester Álvarez-Benedicto, Lukas Farbiak, Martha Márquez Ramírez, Xu Wang, Lindsay T. Johnson, Osamah Mian, Erick D. Guerrero, and Daniel J. Siegwart..* Biomaterials Science, 10, 549-559 (2022).

68. “Lipid nanoparticle chemistry determines how nucleoside base modifications alter mRNA delivery.” Jilian R. Melamed, Khalid A. Hajj, Namit Chaudhary, Daria Strelkova, Mariah L. Arral, Norbert Pardi, Mohamad-Gabriel Alameh, Jason B. Miller, Lukas Farbiak, Daniel J. Siegwart, Drew Weissman, and Kathryn A. Whitehead.* Journal of Controlled Release, 341, 206-214 (2022).

67. “Hydrophobic optimization of functional poly(TPAE-co-suberoyl chloride) for extrahepatic mRNA delivery following intravenous administration.” Xueliang Yu, Shuai Liu, Qiang Cheng, Sang M. Lee, Tuo Wei, Di Zhang, Lukas Farbiak, Lindsay T. Johnson, Xu Wang, and Daniel J. Siegwart.* Pharmaceutics, 13, 1914 (2021).

66. “Recent advances in the targeted fluorescent probes for the detection of metastatic bone cancer.”  Peng Wu, Daniel J. Siegwart, and Hu Xiong.* Science China Chemistry, 64, 1283–1296 (2021).

65. “All-in-one dendrimer-based lipid nanoparticles enable precise HDR-mediated gene editing in vivo.”  Lukas Farbiak, Qiang Cheng, Tuo Wei, Ester Álvarez-Benedicto, Lindsay T. Johnson, Sang Lee, and Daniel J. Siegwart.* Advanced Materials, 33, 2006619 (2021).

64. “TRIM7 inhibits enterovirus replication and promotes emergence of a viral variant with increased pathogenicity.”  Wenchun Fan, Katrina B. Mar, Levent Sari, Ilona K. Gaszek, Qiang Cheng, Bret M. Evers, John M. Shelton, Mary Wight-Carter, Daniel J. Siegwart, Milo M.Lin, John W. Schoggins.* Cell, 184, 3410-3425 (2021).

63. “Membrane-destabilizing ionizable phospholipids for organ-selective mRNA delivery and CRISPR–Cas gene editing.”  Shuai Liu , Qiang Cheng , Tuo Wei , Xueliang Yu, Lindsay T. Johnson , Lukas Farbiak, and Daniel J. Siegwart.* Nature Materials, 20, 701-710 (2021).

• Highlighted in News & Views “Overcoming delivery barriers with LNPs” Nature Materials, 20, 575-577 (2021).

62. “A systematic study of unsaturation in lipid nanoparticles leads to improved mRNA transfection in vivo.”  Sang M. Lee, Qiang Cheng, Xueliang Yu, Shuai Liu, Lindsay T. Johnson, and Daniel J. Siegwart.* Angewandte Chemie International Edition, 60, 5848-5853 (2021).

61. “Delivery of tissue-targeted scalpels: Opportunities and challenges for in vivo CRISPR/Cas-based genome editing.”  Tuo Wei, Qiang Cheng, Lukas Farbiak, Daniel G. Anderson,* Robert Langer,* and Daniel J. Siegwart.*  ACS Nano, 14, 9243–9262 (2020).

60. “Theranostic dendrimer-based lipid nanoparticles containing PEGylated BODIPY dyes for tumor imaging and systemic mRNA delivery in vivo.”  Hu Xiong,* Shuai Liu, Tuo Wei, Qiang Cheng, and Daniel J. Siegwart.*  Journal of Controlled Release, 325, 198-205 (2020).

59. “Systemic nanoparticle delivery of CRISPR-Cas9 ribonucleoproteins for effective tissue specific genome editing.”  Tuo Wei, Qiang Cheng, Yi-Li Min, Eric N. Olson, and Daniel J. Siegwart.*  Nature Communications, 11, 3232 (2020).

58. “Selective organ targeting (SORT) nanoparticles for tissue-specific mRNA delivery and CRISPR–Cas gene editing.” Qiang Cheng, Tuo Wei, Lukas Farbiak, Lindsay T. Johnson, Sean A. Dilliard, and Daniel J. Siegwart.*  Nature Nanotechnology, 15, 313–320 (2020).

• Highlighted in a UT Southwestern press release entitled “SORTing gene editing tools to where they’re needed.” (April 6, 2020)
• Highlighted in News & Views “Nanotechnology for organ-tunable gene editing” Nature Nanotechnology, 15, 253-255 (2020).
• Highlighted in “Seven technologies to watch in 2022” Nature, 601, 658-661 (2022).

57. “The hydrophobic domain structure of linear-dendritic poly(ethylene glycol) lipids affects RNA delivery of lipid nanoparticles.”  Kejin Zhou, Lindsay T. Johnson, Hu Xiong, Sergio Barrios, Jonathan T. Minnig, Yunfeng Yan, Bethanie Abram, Xueliang Yu, and Daniel J. Siegwart.*Molecular Pharmaceutics, 17, 1575-1585 (2020).

56. “PEI fluorination reduces toxicity and promotes liver-targeted siRNA delivery.”  Lian Xue, Yunfeng Yan, Petra Kos, Xiaoping Chen, and Daniel J. Siegwart.*  Drug Delivery and Translational Research, 11, 255-260 (2021).

55. “Lipid-modified aminoglycosides for mRNA delivery to the liver.” Xueliang Yu, Shuai Liu, Qiang Cheng, Tuo Wei, Sang Lee, Di Zhang, and Daniel J. Siegwart.*  Advanced Healthcare Materials, 9, 1901487 (2020).

54. “Strategies, design, and chemistry in siRNA delivery systems.” Yizhou Dong,* Daniel J. Siegwart,* and Daniel G. Anderson.*  Advanced Drug Delivery Reviews, 144, 133-147 (2019).

53. “Dendrimer‐based lipid nanoparticles deliver therapeutic FAH mRNA to normalize liver function and extend survival in a mouse model of Hepatorenal Tyrosinemia Type I.” Qiang Cheng, Tuo Wei, Yuemeng Jia, Lukas Farbiak, Kejin Zhou, Shuyuan Zhang, Yonglong Wei, Hao Zhu, and Daniel J. Siegwart.*  Advanced Materials, 30, 1805308 (2018).

52. “Degradable redox-responsive disulfide-based nanogel drug carriers via dithiol oxidation polymerization.” Sussana A. Elkassih, Petra Kos, Hu Xiong, and Daniel J. Siegwart.* Biomaterials Science, 7, 607-617 (2019).

51. “Adjuvant effect of the novel TLR1/TLR2 agonist Diprovocim synergizes with anti–PD-L1 to eliminate melanoma in mice.” Ying Wang, Lijing Su, Matthew D. Morin, Brian T. Jones, Yuto Mifune, Hexin Shi, Kuan-wen Wang, Xiaoming Zhan, Aijie Liu, Jianhui Wang, Xiaohong Li, Miao Tang, Sara Ludwig, Sara Hildebrand, Kejin Zhou, Daniel J. Siegwart, Eva Marie Y. Moresco, Hong Zhang, Dale L. Boger, and Bruce Beutler.* Proceedings of the National Academy of Sciences, U.S.A., 115, E8698-E8706 (2018).

50. “Design of synthetic materials for intracellular delivery of RNAs: From siRNA-mediated gene silencing to CRISPR/Cas gene editing.” Jason B. Miller and Daniel J. Siegwart.* Nano Research, 11, 5310-5337 (2018).

49. “Tumor-activated water-soluble photosensitizers for near-infrared photodynamic cancer therapy.” Hu Xiong, Kejin Zhou, Yunfeng Yan, Jason B. Miller, and Daniel J. Siegwart.* ACS Applied Materials & Interfaces, 10, 16335-16343 (2018).

48. “The polyploid state plays a tumor-suppressive role in the liver.” Shuyuan Zhang, Kejin Zhou, Xin Luo, Lin Li, Ho-Chou Tu, Alfica Sehgal, Liem H. Nguyen, Yu Zhang, Purva Gopal, Branden D. Tarlow, Daniel J. Siegwart, and Hao Zhu.* Developmental Cell, 44, 447-459 (2018).

47. “Theory study and present CT trend of organic charge transfer complexes.” Atsushi Nagai* and Daniel J. Siegwart. Conjugated Objects: Developments, Synthesis, and Applications. Singapore: Pan Stanford Publishing, 381-412 (2017) (book chapter).

46. “Development of Cationic quaternary ammonium Sulfonamide Amino Lipids (CSALs) for nucleic acid delivery.” Jason B. Miller, Petra Kos, Victor Tieu, Kejin Zhou, and Daniel J. Siegwart.* ACS Applied Materials & Interfaces, 10, 2302-2311 (2018).

45. “Knockdown of anillin actin binding protein blocks cytokinesis in hepatocytes and reduces liver tumor development in mice without affecting regeneration.” Shuyuan Zhang, Liem H. Nguyen, Kejin Zhou, Ho-Chou Tu, Alfica Sehgal, Ibrahim Nassour, Lin Li, Purva Gopal, Joshua Goodman, Amit G. Singal, Adam Yopp, Yu Zhang, Daniel J. Siegwart, and Hao Zhu* Gastroenterology, 154, 1421-1434 (2018).

44. “Translational research to enable personalized treatment of cystic fibrosis.” Marne C. Hagemeijer, Daniel J. Siegwart, Lisa J. Strug, Liudmila Cebotaru, Michael J. Torres, Aderonke Sofoluwe, Jeffrey M. Beekman* Journal of Cystic Fibrosis, 17, S46–S51 (2018).

43. “Systemic mRNA delivery to the lungs by functional polyester-based carriers.” Yunfeng Yan, Hu Xiong, Xinyi Zhang, Qiang Cheng, and Daniel J. Siegwart* Biomacromolecules, 18, 4307-4315 (2017).

42. “High-contrast fluorescence detection of metastatic breast cancer including bone and liver micrometastases via size-controlled pH-activatable water-soluble probes.” Hu Xiong, Hao Zuo, Yunfeng Yan, Gino Occhialini, Kejin Zhou, Yihong Wan, and Daniel J. Siegwart* Advanced Materials, 29, 1700131 (2017).

41. “Non-viral CRISPR/Cas gene editing in vitro and in vivo enabled by synthetic nanoparticle co-delivery of Cas9 mRNA and sgRNA.” Jason B. Miller, Shuyuan Zhang, Petra Kos, Hu Xiong, Kejin Zhou, Sofya S. Perelman, Hao Zhu,  and Daniel J. Siegwart* Angewandte Chemie International Edition, 56, 1059-1063 (2017).  (Published online December 16, 2016.)

• Highlighted in C&EN’s cover story on CRISPR/Cas delivery entitled “CRISPR’s breakthrough problem: If the CRISPR gene editing system is to live up to its disease-curing potential, researchers must devise a plan to deliver it into the body” (February 13, 2017)
• Received the Very Important Paper (VIP) designation by Angew. Chem.
• Highlighted in a Timeline of key advances for mRNA therapeutics and delivery in “Tools for translation: Non-viral materials for therapeutic mRNA delivery.”

40. “HDAC inhibitor conjugated polymeric prodrug micelles for doxorubicin delivery.” Suchithra A. Senevirathne, Katherine E. Washington, Jason B. Miller, Michael C. Biewer, David Oupicky,* Daniel J. Siegwart,* and Mihaela C. Stefan* Journal of Materials Chemistry B, 5, 2106-2114 (2017).

39. “Gpr132 sensing of lactate mediates tumor-macrophage interplay to promote breast cancer metastasis.” Peiwen Chen, Hao Zuo, Hu Xiong, Matthew J. Kolar, Qian Chu, Alan Saghatelian, Daniel J. Siegwart, and Yihong Wan.* Proceedings of the National Academy of Sciences, U.S.A., 114, 580-585 (2017).

38. “Aerosol delivery of stabilized polyester-siRNA nanoparticles to silence gene expression in orthotopic lung tumors.” Yunfeng Yan, Kejin Zhou, Hu Xiong, Jason B. Miller, Edward A. Motea, David A. Boothman, Li Liu,* and Daniel J. Siegwart*  Biomaterials, 118, 84-93 (2017).

37. “Functional polyesters enable selective siRNA delivery to lung cancer over matched normal cells.” Yunfeng Yan, Li Liu, Hu Xiong, Jason B. Miller, Kejin Zhou, Petra Kos, Kenneth E. Huffman, Sussana Elkassih, John W. Norman, Ryan Carstens, James Kim, John D. Minna, and Daniel J. Siegwart.*  Proceedings of the National Academy of Sciences, U.S.A., 113, E5702–E5710 (2016)

• Highlighted in a UT Southwestern press release entitled “Drug-loaded synthetic nanoparticles can distinguish lung cancer cells from healthy cells.” (September 14, 2016)
• Highlighted in Carnegie Mellon Today, as a part of an alumni profile story entitled “Cancer Breakthrough” (December 6, 2016)

36. “Intercalation-mediated nucleic acid nanoparticles for siRNA delivery.“ Kejin Zhou, Petra Kos, Yunfeng Yan, Hu Xiong, Yi-Li Min, Jonathan T. Minnig, Jason B. Miller, and Daniel J. Siegwart.* Chemical Communications, 52, 12155-12158 (2016).

35. “Progress towards the synthesis of amino polyesters via ring-opening polymerization (ROP) of functional lactones.” Jing Hao, Sussana Elkassih, and Daniel J. Siegwart.* Synlett, 27, 2285-2292 (2016).

34. “Activatable water soluble probes enhance tumor imaging by responding to dysregulated pH and exhibiting high tumor-to-liver fluorescence emission contrast.” Hu Xiong, Petra Kos, Yunfeng Yan, Kejin Zhou, Jason B. Miller, Sussana Elkassih, and Daniel J. Siegwart* Bioconjugate Chemistry, 27, 1737–1744 (2016).

33. “Modular degradable dendrimers enable small RNAs to extend survival in an aggressive liver cancer model.” Kejin Zhou, Liem H. Nguyen, Jason B. Miller, Yunfeng Yan, Petra Kos, Hu Xiong, Lin Li, Jing Hao, Jonathan T. Minnig, Hao Zhu, and Daniel J. Siegwart.* Proceedings of the National Academy of Sciences, U.S.A., 113, 520-525 (2016).

• Highlighted in a UT Southwestern press release entitled “Scientists synthesize nanoparticles that can deliver tumor suppressors to damaged livers.” (January 25, 2016)
• Highlighted in the UT Southwestern Annual Review 2016: “Breaking new ground in research and treatment.” (February 7, 2017)

32. “Tumor imaging based on photon upconversion of Pt(II) porphyrin rhodamine co-modified NIR excitable cellulose enhanced by aggregation.” Atsushi Nagai,* Jason B. Miller, Petra Kos, Sussana Elkassih, Hu Xiong, and Daniel J. Siegwart*  ACS Biomaterials Science & Engineering, 1, 1206-1210 (2015).

31. “Precise regulation of let-7 levels balance organ regeneration against tumor suppression.”  Linwei Wu, Liem H. Nguyen, Kejin Zhou, Yvanka de Soysa, Lin Li, Jason B. Miller, Jianmin Tian, Joseph Locker, Shuyuan Zhang, Gen Shinoda, Marc T. Seligson, Lauren R. Zeitels, Asha Acharya, Sam C. Wang, Joshua T. Mendell, Jinsuke Nishino, Sean J. Morrison, Daniel J. Siegwart, George Q. Daley, Ng Shyh-Chang, and Hao Zhu.* eLife, 4, e09431 (2015). 

30.  “Rapid synthesis of a lipocationic polyester library via ring-opening polymerization of functional valerolactones for efficacious siRNA delivery.”  Jing Hao, Petra Kos, Kejin Zhou, Jason B. Miller, Lian Xue, Yunfeng Yan, Hu Xiong, Sussana Elkassih, and Daniel J. Siegwart.*  Journal of the American Chemical Society, 137, 9206-9209 (2015).

29. “Biocompatible organic charge transfer complex nanoparticles based on a semi-crystalline cellulose template.”  Atsushi Nagai,* Jason B. Miller, Jia Du, Petra Kos, Mihaela C. Stefan, and Daniel J. Siegwart.*  Chemical Communications, 15, 11868-11871 (2015).

28. “One-pot synthesis of functional poly(amino ester sulfide)s and utility in delivering pDNA and siRNA.”  Yunfeng Yan, Lian Xue, Jason B. Miller, Kejin Zhou, Petra Kos, Sussana Elkassih, Atsushi Nagai, Hu Xiong, and Daniel J. Siegwart.*  Polymer, 72, 271-280 (2015).

27. “Fine-tuning thermoresponsive functional poly(ε-caprolactone)s to enhance micelle stability and drug loading.”  Elizabeth A. Rainbolt, Jason B. Miller, Katherine E. Washington, Suchithra A. Senevirathne, Michael C. Biewer, Daniel J. Siegwart,* Mihaela C. Stefan.*  Journal of Materials Chemistry B, 3, 1779-1787 (2015).

26. “Polymers for drug delivery.” Daniel J. Siegwart.* McGraw-Hill 2015 Yearbook of Science & Technology. New York: McGraw-Hill, (2015) (book chapter).

25. “Scalable synthesis and derivation of functional polyesters bearing ene and epoxide side chains.” Yunfeng Yan, Daniel J. Siegwart.* Polymer Chemistry, 5, 1362-1371 (2014).

Siegwart, DJ: Postdoctoral research at MIT.

24. “Lipopeptide nanoparticles for potent and selective siRNA delivery in rodents and nonhuman primates.” Yizhou Dong, Kevin T. Love, J. Robert Dorkin, Sasilada Sirirungruang, Yunlong Zhang, Delai Chen, Roman L. Bogorad, Hao Yin, Yi Chen, Arturo J. Vegas, Christopher A. Alabi, Gaurav Sahay, Karsten T. Olejnik, Weiheng Wang, Avi Schroeder, Abigail K. R. Lytton-Jean, Daniel J. Siegwart, Akin Akinc, Carmen Barnes, Scott A. Barros, Mary Carioto, Kevin Fitzgerald, Julia Hettinger, Varun Kumar, Tatiana I. Novobrantseva, June Qin, William Querbes, Victor Koteliansky, Robert Langer, Daniel G. Anderson.* Proceedings of the National Academy of Sciences, 2014, 111, 3955-3960.

23. “Biophysical properties of degradable polymeric nanoparticles: Effect of molecular weight of amine end-modified poly(ß-amino ester)s on gene delivery efficiency and toxicity.” Ahmed A. Eltoukhy, Daniel J. Siegwart, Christopher A. Alabi, Jay S. Rajan, Robert Langer, Daniel G. Anderson.* Biomaterials, 2012, 33, 3594-3603.

22. “Automated ARGET ATRP accelerates catalyst optimization for the synthesis of thiol-functionalized polymers.” Daniel J. Siegwart, Matthias Leiendecker, Robert Langer, Daniel G. Anderson.* Macromolecules, 2012, 45, 1254-1261.

21. “Combinatorial synthesis of chemically diverse core-shell nanoparticles for intracellular delivery.” Daniel J. Siegwart, Kathryn A. Whitehead, Lutz Nuhn, Gaurav Sahay, Hao Cheng, Shan Jiang, Minglin Ma, Abigail Lytton-Jean, Arturo Vegas, Patrick Fenton, Christopher G. Levins, Kevin T. Love, Haeshin Lee, Christina Cortez, Sean P. Collins, Ying Fei Li, Janice Jang, William Querbes, Christopher Zurenko, Tatiana Novobrantseva, Robert Langer, Daniel G. Anderson.* Proceedings of the National Academy of Sciences, 2011, 108, 12996-13001.

20. “Development of cationic polymer coatings to regulate foreign-body responses.” Minglin Ma, Wendy F. Liu, Paulina S. Hill, Kaitlin M. Bratlie, Daniel J. Siegwart, Justin Chin, Miri Park, Joao Guerreiro, Daniel G. Anderson.* Advanced Materials, 2011, 23, H189-H194.

19. “A novel family of biodegradable poly(ester amide) elastomers.” Hao Cheng, Paulina S. Hill, Daniel J. Siegwart, Nathaniel Vacanti, Abigail K. R. Lytton-Jean, Seung-Woo Cho, Anne Ye, Robert Langer, Daniel G. Anderson.* Advanced Materials, 2011, 12, H95-H100.

18. “An injectable thiol-acrylate poly(ethylene glycol) hydrogel.” Christopher D. Pritchard, Timothy M. O’Shea, Daniel J. Siegwart, Eliezer Calo, Daniel G. Anderson, Francis M. Reynolds, John A. Thomas, Jonathan R. Slotkin, Eric J. Woodard, Robert Langer.* Biomaterials, 2010, 32, 587-597.

17. “Nanoparticulate cellular patches for cell-mediated tumoritropic delivery.” Hao Cheng, Christian J. Kastrup, Renuka Ramanathan, Daniel J. Siegwart, Minglin Ma, Said R. Bogatyrev, Qiaobing Xu, Kathryn A. Whitehead, Robert Langer, Daniel G. Anderson.* ACS Nano, 2010, 4, 625-631.

Siegwart, DJ: Graduate research at CMU.

16. “ATRP in the design of functional materials for biomedical applications.” Daniel J. Siegwart, Jung Kwon Oh, Krzysztof Matyjaszewski.* Progress in Polymer Science, 2012, 37, 18-37.

15. “Preparation of Cationic Nanogels for Nucleic Acid Delivery.” Saadyah E. Averick, Eduardo Paredes Ainara Irastorza, Arun R. Shrivats, Abiraman Srinivasan, Daniel J. Siegwart, Andrew J. Magenau, Hong Y. Cho, Eric Hsu, Amram A. Averick, Jinku Kim, Shiguang Liu, Jeffrey O. Hollinger, Subha R. Das, Krzysztof Matyjaszewski.* Biomacromolecules, 2012, 13, 3445-3449.

14. “Rapid cellular internalization of multifunctional star polymers prepared by Atom Transfer Radical Polymerization.” Hong Y. Cho, Haifeng Gao, Abiraman Srinivasan, Joanna Hong, Sidi A. Bencherif, Daniel J. Siegwart, Hyun-jong Paik, Jeffrey O. Hollinger, Krzysztof Matyjaszewski.* Biomacromolecules, 2010, 11, 2199-2203.

13. “Nanostructured hybrid hydrogels prepared by a combination of atom transfer radical polymerization and free radical polymerization.” Sidi A. Bencherif, Daniel J. Siegwart, Abiraman Srinivasan, Ferenc Horkay, Jeffrey O. Hollinger, Newell R. Washburn, Krzysztof Matyjaszewski.* Biomaterials, 2009, 30, 5270-5278.

12. “Cellular uptake of functional nanogels prepared by inverse miniemulsion ATRP with encapsulated proteins, carbohydrates, and gold nanoparticles.” Daniel J. Siegwart, Abiraman Srinivasan, Sidi A. Bencherif, Anuradha Karunanidhi, Jung Kwon Oh, Swaroopa Vaidya, Rongchao Jin, Jeffrey O. Hollinger, Krzysztof Matyjaszewski.* Biomacromolecules, 2009, 10, 2300-2309.

11. “Cell-adhesive star polymers prepared by ATRP.” Sidi A. Bencherif, Haifeng Gao, Abiraman Srinivasan, Daniel J. Siegwart, Jeffrey O. Hollinger,* Newell R. Washburn,* Krzysztof Matyjaszewski.* Biomacromolecules, 2009, 10, 1795-1803.

10. “The development of microgels/nanogels for drug delivery applications.” Jung Kwon Oh, Ray Drumright, Daniel J. Siegwart, Krzysztof Matyjaszewski.* Progress in Polymer Science, 2008, 33, 448-477.

9. “Biotin-, pyrene- and GRGDS-functionalized ATRP polymers and nanogels via end group modification.” Daniel J. Siegwart, Jung Kwon Oh, Haifeng Gao, Sidi A. Bencherif, Fabien Perineau, Andrew K. Bohaty, Jeffrey O. Hollinger, Krzysztof Matyjaszewski.* Macromolecular Chemistry and Physics, 2008, 209, 2179-2193.

8. “Synthesis, characterization, and in vitro cell culture viability of degradable poly(N-isopropylacrylamide-co-5,6-benzo-2-methylene-1,3-dioxepane)-based polymers and crosslinked gels.” Daniel J. Siegwart, Sidi A. Bencherif, Abiraman Srinivasan, Jeffrey O. Hollinger, Krzysztof Matyjaszewski.* Journal of Biomedical Materials Research Part A, 2008, 87, 345-358.

7. “Synthesis and biodegradation of nanogels as delivery carriers for carbohydrate drugs.” Jung Kwon Oh, Daniel J. Siegwart, Krzysztof Matyjaszewski.* Biomacromolecules, 2007, 8, 3326-3331.

6. “Biodegradable nanogels prepared by atom transfer radical polymerization as potential drug delivery carriers: synthesis, biodegradation, in vitro release, and bioconjugation.” Jung Kwon Oh, Daniel J. Siegwart, Hyung-il Lee, Gizelle Sherwood, Linda Peteanu, Jeffrey O. Hollinger, Kazunori Kataoka, Krzysztof Matyjaszewski.* Journal of the American Chemical Society, 2007, 129, 5939-5945.

5. “Solvent induced morphologies of poly(methyl methacrylate-b-ethylene oxide-b-methyl methacrylate) triblock copolymers synthesized by atom transfer radical polymerization.” Daniel J. Siegwart, Wei Wu, Monisha Mandalaywala, Magi Tamir, Traian Sarbu, Michael S. Silverstein, Tomasz Kowalewski, Jeffrey O. Hollinger, Krzysztof Matyjaszewski.* Polymer, 2007, 48, 7279-7290.

4. “Synthesis and characterization of styrene/butyl acrylate linear and star block copolymers via Atom Transfer Radical Polymerization.” Jinyu Huang, Shijun Jia, Daniel J. Siegwart, Tomasz Kowalewski, Krzysztof Matyjaszewski.* Macromolecular Chemistry and Physics, 2006, 207, 801-811.

3. “Characterization of α-,ω-dihydroxypolystyrene by gradient polymer elution chromatography and two-dimensional liquid chromatography.” Haifeng Gao, Daniel J. Siegwart, Nazeem Jahed, Traian Sarbu, Krzysztof Matyjaszewski.* Designed Monomers and Polymers, 2005, 8, 533-546.

2. “Synthesis of hydroxy-telechelic poly(methyl acrylate) and polystyrene by Atom Transfer Radical Coupling.” Traian Sarbu, Koon-Yee Lin, James Spanswick, Roberto R. Gil, Daniel J. Siegwart, Krzysztof Matyjaszewski.* Macromolecules, 2004, 37, 9694-9700.

1. “Polystyrene with designed molecular weight distribution by Atom Transfer Radical Coupling.” Traian Sarbu, Koon-Yee Lin, John Ell, Daniel J. Siegwart, James Spanswick, Krzysztof Matyjaszewski.* Macromolecules, 2004, 37, 3120-3127.